Hotspot

ndhF-rpl32

SSC · 116610-117690 · 1081 bp

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Pi 0.0063
MarkerSeek score 62.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1081
variable_sites
19
pi
0.0063
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 40.0 50.0 55.3 58.3 1250-1310 bp 1.0 61.0
ndhF-rpl32_p2 TGTTGCTGTCGGAATAAGTAGA GGTGGAAACCGATTTCGCTA 40.9 50.0 57.2 58.3 1249-1309 bp 1.0 60.3
ndhF-rpl32_p3 CTGGAAGTGGAAGAAAAGGT GGTGGAAACCGATTTCGCTA 45.0 50.0 55.2 58.3 1202-1262 bp 1.0 60.2
ndhF-rpl32_p4 TTTGTTGCTGTCGGAATAAGT GGTGGAAACCGATTTCGCTA 38.1 50.0 56.1 58.3 1251-1311 bp 1.0 59.4
ndhF-rpl32_p5 TGCTGTCGGAATAAGTAGAAGTC GGTGGAAACCGATTTCGCTA 43.5 50.0 58.0 58.3 1246-1306 bp 1.0 58.6