Hotspot

petA-psbJ

LSC · 65915-67022 · 1108 bp

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Pi 0.00347
MarkerSeek score 50.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1108
variable_sites
12
pi
0.00347
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC TCTGGGTTGGGTTCATCTCT 50.0 50.0 57.9 58.0 1234-1252 bp 1.0 80.0
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC TGGGTTGGGTTCATCTCTCT 50.0 50.0 57.9 58.0 1232-1250 bp 1.0 80.0
petA-psbJ_p3 GGCCTTTTGTTCTTCTTCGC CTGGGTTGGGTTCATCTCTC 50.0 55.0 57.9 57.6 1233-1251 bp 1.0 77.1
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT TCTGGGTTGGGTTCATCTCT 50.0 50.0 58.7 58.0 1241-1259 bp 1.0 75.0
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTCT TGGGTTGGGTTCATCTCTCT 50.0 50.0 58.7 58.0 1239-1257 bp 1.0 75.0