Hotspot

trnG-GCC-trnfM-CAU

LSC · 38582-38721 · 140 bp

Back to Puya

Pi 0.0257
MarkerSeek score 58.90
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
140
variable_sites
11
pi
0.0257
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 206 bp 1.0 83.5
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 207 bp 1.0 83.5
trnG-GCC-trnfM-CAU_p3 ATGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.9 58.0 247 bp 1.0 82.5
trnG-GCC-trnfM-CAU_p4 GTGTGGACATATACGTGCGT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 283 bp 1.0 82.1
trnG-GCC-trnfM-CAU_p5 TGTGTGGACATATACGTGCG CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 284 bp 1.0 82.1