Hotspot

psbZ-trnG-GCC

LSC · 38182-38510 · 329 bp

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Pi 0.00122
MarkerSeek score 27.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
329
variable_sites
2
pi
0.00122
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
27.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 AACATCCCAGATGCGAATCC AGAAGGGCCCTCTTTACCAT 50.0 50.0 58.0 58.0 1171-1172 bp 1.0 80.9
psbZ-trnG-GCC_p2 AACATCCCAGATGCGAATCC AGGGCCCTCTTTACCATTCT 50.0 50.0 58.0 58.0 1168-1169 bp 1.0 80.9
psbZ-trnG-GCC_p3 CTAGCCGAGCCATTCCTTTT AGGGCCCTCTTTACCATTCT 50.0 50.0 58.0 58.0 1407-1409 bp 1.0 80.9
psbZ-trnG-GCC_p4 AACATCCCAGATGCGAATCC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.0 58.0 1024-1025 bp 1.0 80.9
psbZ-trnG-GCC_p5 CTAGCCGAGCCATTCCTTTT AGAAGGGCCCTCTTTACCAT 50.0 50.0 58.0 58.0 1410-1412 bp 1.0 80.8