Hotspot

trnS-UGA-psbZ

LSC · 37664-37992 · 329 bp

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Pi 0.005
MarkerSeek score 35.20
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
329
variable_sites
4
pi
0.005
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CGGTTTTCAAGACCAGAGCT ACCAACCATCAGAAGAAGCA 50.0 45.0 58.1 57.0 472 bp 1.0 72.3
trnS-UGA-psbZ_p2 CTATCAACCACTCGGCCATC ACCAACCATCAGAAGAAGCA 55.0 45.0 58.1 57.0 454 bp 1.0 72.0
trnS-UGA-psbZ_p3 CAGAGCTATCAACCACTCGG ACCAACCATCAGAAGAAGCA 55.0 45.0 57.8 57.0 459 bp 1.0 71.5
trnS-UGA-psbZ_p4 CCAGAGCTATCAACCACTCG ACCAACCATCAGAAGAAGCA 55.0 45.0 57.8 57.0 460 bp 1.0 71.5
trnS-UGA-psbZ_p5 ACCGGTTTTCAAGACCAGAG ACCAACCATCAGAAGAAGCA 50.0 45.0 57.7 57.0 474 bp 0.1 34.3