Hotspot

trnT-GGU-psbD

LSC · 33958-35001 · 1044 bp

Back to Puya

Pi 0.00392
MarkerSeek score 45.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1044
variable_sites
14
pi
0.00392
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 50.0 50.0 57.9 58.1 1217-1225 bp 1.0 79.4
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1207-1215 bp 1.0 78.3
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1180-1188 bp 1.0 78.0
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA CAAAAACGAAACGGTCCCTC 50.0 50.0 57.9 57.6 1206-1214 bp 1.0 77.1
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.3 58.2 1170-1178 bp 1.0 76.9