Hotspot

trnS-GCU-trnR-UCU

LSC · 8697-11049 · 2353 bp

Back to Puya

Pi 0.00514
MarkerSeek score 47.90
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
2353
variable_sites
36
pi
0.00514
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 GAGAAAGCGGAAAGAGAGGG AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.0 56.9 2484-2576 bp 1.0 66.0
trnS-GCU-trnR-UCU_p2 ATTAGCAATCCGCCGCTTTA AGAAGACCTCTGTCCTATCCA 45.0 47.6 58.0 56.9 2429-2521 bp 1.0 65.8
trnS-GCU-trnR-UCU_p3 GAGAAAGCGGAAAGAGAGGG AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.0 57.9 2490-2582 bp 1.0 65.3
trnS-GCU-trnR-UCU_p4 ATTAGCAATCCGCCGCTTTA AGGTTTAGAAGACCTCTGTCCT 45.0 45.5 58.0 57.9 2435-2527 bp 1.0 65.2
trnS-GCU-trnR-UCU_p5 AGAAAGCGGAAAGAGAGGGA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.4 56.9 2483-2575 bp 1.0 63.3