Hotspot

trnK-UUU-rps16

LSC · 4075-4773 · 699 bp

Back to Puya

Pi 0.0065
MarkerSeek score 54.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
699
variable_sites
11
pi
0.0065
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCTCAACCTACGGGAACTG 50.0 57.9 58.2 57.9 804-806 bp 1.0 70.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCTCAACCTACGGGAACTG 50.0 57.9 58.2 57.9 803-805 bp 1.0 70.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GTGCTCAACCTACGGGAAC 50.0 57.9 58.2 58.2 806-808 bp 1.0 70.7
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GTGCTCAACCTACGGGAAC 50.0 57.9 58.2 58.2 805-807 bp 1.0 70.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGCTCAACCTACGGGAACT 50.0 52.6 58.2 58.2 804-806 bp 1.0 70.1