Hotspot

ycf1

SSC · 128076-133745 · 5670 bp

Back to Purshia

Pi 0.00141

MarkerSeek score 46.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 8
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTTTTACGTCGTTGAGCAC	ACCGAGGAAAGTGTGGTAGA	45.0	50.0	57.0	58.0	222 bp	1.0	72.7
ycf1_p2	TGTTTTACGTCGTTGAGCAC	GAAACCGAGGAAAGTGTGGT	45.0	50.0	57.0	58.0	225 bp	1.0	72.4
ycf1_p3	CTGTTTTACGTCGTTGAGCAC	ACCGAGGAAAGTGTGGTAGA	47.6	50.0	58.1	58.0	223 bp	1.0	71.8
ycf1_p4	CTGTTTTACGTCGTTGAGCAC	GAAACCGAGGAAAGTGTGGT	47.6	50.0	58.1	58.0	226 bp	1.0	71.5
ycf1_p5	TGTTTTACGTCGTTGAGCAC	TGGAACAACAGGAGGGAGAA	45.0	50.0	57.0	58.2	242 bp	1.0	71.2