Hotspot

matK

LSC · 2079-3590 · 1512 bp

Back to Purshia

Pi 0.00397

MarkerSeek score 61.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 6
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	TTTCCATTTCCACGGTCACA	ACCTTGTTTTGACTGTATCGT	45.0	38.1	57.6	55.5	1631 bp	1.0	49.2
matK_p2	TTCCATTTCCACGGTCACAT	ACCTTGTTTTGACTGTATCGT	45.0	38.1	57.4	55.5	1630 bp	1.0	47.4
matK_p3	TTCCACGGTCACATAACCAA	ACCTTGTTTTGACTGTATCGT	45.0	38.1	57.1	55.5	1624 bp	1.0	44.5
matK_p4	TTTCCACGGTCACATAACCA	ACCTTGTTTTGACTGTATCGT	45.0	38.1	57.1	55.5	1625 bp	1.0	44.5
matK_p5	TTTCCATTTCCACGGTCACA	ACCTTGTTTTGACTGTATCGTACT	45.0	37.5	57.6	58.1	1631 bp	1.0	44.3