Hotspot

trnV-GAC-rps12

IRa · 142713-144415 · 1703 bp

Back to Purshia

Pi 0.0053
MarkerSeek score 61.70
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1703
variable_sites
9
pi
0.0053
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1810-1815 bp 1.0 78.7
trnV-GAC-rps12_p2 ATGACTTCCACCACGTCAAG GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1809-1814 bp 1.0 78.7
trnV-GAC-rps12_p3 AACTGATGACTTCCACCACG GAGGTTAAGCGTACTCTGGC 50.0 55.0 57.8 58.1 1814-1819 bp 1.0 78.6
trnV-GAC-rps12_p4 GATGACTTCCACCACGTCAA GGTTAAGCGTACTCTGGCAA 50.0 50.0 57.8 57.9 1808-1813 bp 1.0 78.6
trnV-GAC-rps12_p5 ATGACTTCCACCACGTCAAG GGTTAAGCGTACTCTGGCAA 50.0 50.0 57.8 57.9 1807-1812 bp 1.0 78.5