Hotspot

ndhD-psaC

SSC · 121115-121247 · 133 bp

Back to Purshia

Pi 0.0156

MarkerSeek score 65.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 133
variable_sites: 2
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AAAATTAAGGAACCCGCGGA	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.8	57.7	286-291 bp	1.0	77.7
ndhD-psaC_p2	AAAATTAAGGAACCCGCGGA	CAGAGGACTGTGTTGGTTGT	45.0	50.0	57.8	57.7	306-311 bp	1.0	77.5
ndhD-psaC_p3	AAAATTAAGGAACCCGCGGA	GAGATGTGAATCCGCCTGTC	45.0	55.0	57.8	58.4	284-289 bp	1.0	76.9
ndhD-psaC_p4	AAAATTAAGGAACCCGCGGA	GCCTGTCCAACGGATTTCTT	45.0	50.0	57.8	58.5	271-276 bp	1.0	76.7
ndhD-psaC_p5	AAATTAAGGAACCCGCGGAT	AAGAGATGTGAATCCGCCTG	45.0	50.0	57.6	57.7	285-290 bp	1.0	75.9