Hotspot

rpl32-trnL-UAG

SSC · 117279-118179 · 901 bp

Back to Purshia

Pi 0.00225
MarkerSeek score 61.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
901
variable_sites
2
pi
0.00225
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGTTGAAAGCCTTTTCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1000-1014 bp 1.0 79.9
rpl32-trnL-UAG_p2 GCGTTGAAAGCCTTTTCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1001-1015 bp 1.0 79.9
rpl32-trnL-UAG_p3 GCGTTGAAAGCCTTTTCGTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1010-1024 bp 1.0 78.9
rpl32-trnL-UAG_p4 GCGTTGAAAGCCTTTTCGTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.2 57.8 1009-1023 bp 1.0 78.4
rpl32-trnL-UAG_p5 GCGTTGAAAGCCTTTTCGTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.2 57.8 1008-1022 bp 1.0 78.4