Hotspot

rps12-trnV-GAC

IRb · 102145-103847 · 1703 bp

Back to Purshia

Pi 0.0053
MarkerSeek score 61.70
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1703
variable_sites
9
pi
0.0053
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 GAGGTTAAGCGTACTCTGGC GATGACTTCCACCACGTCAA 55.0 50.0 58.1 57.8 1810-1815 bp 1.0 78.7
rps12-trnV-GAC_p2 GAGGTTAAGCGTACTCTGGC ATGACTTCCACCACGTCAAG 55.0 50.0 58.1 57.8 1809-1814 bp 1.0 78.7
rps12-trnV-GAC_p3 GAGGTTAAGCGTACTCTGGC AACTGATGACTTCCACCACG 55.0 50.0 58.1 57.8 1814-1819 bp 1.0 78.6
rps12-trnV-GAC_p4 GGTTAAGCGTACTCTGGCAA GATGACTTCCACCACGTCAA 50.0 50.0 57.9 57.8 1808-1813 bp 1.0 78.6
rps12-trnV-GAC_p5 GGTTAAGCGTACTCTGGCAA ATGACTTCCACCACGTCAAG 50.0 50.0 57.9 57.8 1807-1812 bp 1.0 78.5