Hotspot

accD-psaI

LSC · 61583-62375 · 793 bp

Back to Purshia

Pi 0.0145

MarkerSeek score 70.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 793
variable_sites: 11
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GATCCAATCGTACCGCGTAA	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.9	58.0	938-949 bp	1.0	82.3
accD-psaI_p2	TCCAATCGTACCGCGTAATC	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.9	58.0	936-947 bp	1.0	82.3
accD-psaI_p3	CCAATCGTACCGCGTAATCT	TTGCCGGAAATACTAAGCCC	50.0	50.0	57.9	58.0	935-946 bp	1.0	82.2
accD-psaI_p4	GATCCAATCGTACCGCGTAA	AAATGGAGGGGAAGCTGTTG	50.0	50.0	57.9	58.1	905-916 bp	1.0	82.2
accD-psaI_p5	TCCAATCGTACCGCGTAATC	AAATGGAGGGGAAGCTGTTG	50.0	50.0	57.9	58.1	903-914 bp	1.0	82.2