Hotspot

rbcL-accD

LSC · 59407-60121 · 715 bp

Back to Purshia

Pi 0
MarkerSeek score 62.30
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
715
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGTCCTGAACTAGCTGCTG CGAATCGAACCGCCATTTTT 55.0 45.0 57.7 57.7 798-815 bp 1.0 78.2
rbcL-accD_p2 GAGTCCTGAACTAGCTGCTG ATCGAATCGAACCGCCATTT 55.0 45.0 57.7 58.3 800-817 bp 1.0 77.5
rbcL-accD_p3 GAGTCCTGAACTAGCTGCTG ATCGAACCGCCATTTTTCCA 55.0 45.0 57.7 58.5 795-812 bp 1.0 76.6
rbcL-accD_p4 GAGTCCTGAACTAGCTGCTG TCGAACCGCCATTTTTCCAT 55.0 45.0 57.7 58.5 794-811 bp 1.0 76.6
rbcL-accD_p5 GCTGCTGCTTGTGAAGTATG CGAATCGAACCGCCATTTTT 50.0 45.0 57.5 57.7 785-802 bp 1.0 76.3