Hotspot

psbZ-trnG-GCC

LSC · 38011-38522 · 512 bp

Back to Purshia

Pi 0.0209

MarkerSeek score 70.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 9
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTTGCTTCTCCTGAGGGTTG	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	600-681 bp	1.0	82.4
psbZ-trnG-GCC_p2	TTTGCTTCTCCTGAGGGTTG	GAATCGAACCCGCATCTTCT	50.0	50.0	57.7	58.1	601-682 bp	1.0	82.4
psbZ-trnG-GCC_p3	GGGTTGGTCGGGTAACAAAA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.3	58.1	586-667 bp	1.0	82.2
psbZ-trnG-GCC_p4	GGGTTGGTCGGGTAACAAAA	GAATCGAACCCGCATCTTCT	50.0	50.0	58.3	58.1	587-668 bp	1.0	82.2
psbZ-trnG-GCC_p5	TTTGCTTCTCCTGAGGGTTG	GAACCCGCATCTTCTCCTTG	50.0	55.0	57.7	58.6	596-677 bp	1.0	77.9