Hotspot

rps15

SSC · 126344-126616 · 273 bp

Back to Pulsatilla

Pi 0.000916

MarkerSeek score 22.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 1
pi: 0.000916
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ATGAGTCGAAGAACACCGTG	AGTCGTTTCGGTATGTCAAGG	50.0	47.6	57.9	58.0	962-1114 bp	1.0	79.7
rps15_p2	ATGAGTCGAAGAACACCGTG	GTCGTTTCGGTATGTCAAGGA	50.0	47.6	57.9	58.0	961-1113 bp	1.0	79.7
rps15_p3	TCCGCCATAAAAGGACCAAG	AGTCGTTTCGGTATGTCAAGG	50.0	47.6	57.9	58.0	1247-1399 bp	1.0	78.4
rps15_p4	TCCGCCATAAAAGGACCAAG	GTCGTTTCGGTATGTCAAGGA	50.0	47.6	57.9	58.0	1246-1398 bp	1.0	78.4
rps15_p5	CGTAAGGTAGATCAGCAGCC	AGTCGTTTCGGTATGTCAAGG	55.0	47.6	57.9	58.0	1384-1536 bp	1.0	78.0