Hotspot

ccsA-ndhD

SSC · 118185-118683 · 499 bp

Back to Pulsatilla

Pi 0.115

MarkerSeek score 80.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 499
variable_sites: 112
pi: 0.115
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCTGCAATTGTGGCTTCT	TCAATTGACAAGGTAGAAGCT	40.0	38.1	55.9	54.9	543-750 bp	1.0	52.0
ccsA-ndhD_p2	AATTCTGCAATTGTGGCTTCT	TCAATTGACAAGGTAGAAGCT	38.1	38.1	56.7	54.9	544-751 bp	1.0	52.0
ccsA-ndhD_p3	TGCAATTGTGGCTTCTATAGGA	TCAATTGACAAGGTAGAAGCT	40.9	38.1	57.5	54.9	539-746 bp	1.0	52.0
ccsA-ndhD_p4	ATTCTGCAATTGTGGCTTCT	ATCAATTGACAAGGTAGAAGCT	40.0	36.4	55.9	55.4	544-751 bp	1.0	52.0
ccsA-ndhD_p5	ATTCTGCAATTGTGGCTTCT	TCAATTGACAAGGTAGAAGC	40.0	40.0	55.9	53.2	543-750 bp	1.0	52.0