Hotspot

trnP-UGG-psaJ

LSC · 68734-69160 · 427 bp

Back to Pulsatilla

Pi 0.0268

MarkerSeek score 57.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 21
pi: 0.0268
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GCCACGGAGAGATACGTTTT	50.0	50.0	58.1	58.0	462-535 bp	1.0	86.6
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GCCACGGAGAGATACGTTTT	50.0	50.0	58.1	58.0	461-534 bp	1.0	86.6
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GCCACGGAGAGATACGTTTT	50.0	50.0	58.1	58.0	473-546 bp	1.0	86.5
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GCCACGGAGAGATACGTTTT	50.0	50.0	58.1	58.0	472-545 bp	1.0	86.5
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GCCACGGAGAGATACGTTTT	50.0	50.0	58.1	58.0	475-548 bp	1.0	86.5