Hotspot

trnS-GCU-trnL-UAA

LSC · 48206-48933 · 728 bp

Back to Pulsatilla

Pi 0.0252

MarkerSeek score 65.20

Primer available yes

Label trnS-GCU-trnL-UAA

Feature Metadata

label_name: trnS-GCU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 30
pi: 0.0252
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnL-UAA_p1	CAATCCGACGCTTTAGTCCA	CTACCAATTCCGCCATACCC	50.0	55.0	58.0	58.1	685-785 bp	1.0	86.4
trnS-GCU-trnL-UAA_p2	AATCCGACGCTTTAGTCCAC	CTACCAATTCCGCCATACCC	50.0	55.0	58.0	58.1	684-784 bp	1.0	86.4
trnS-GCU-trnL-UAA_p3	CAATCCGACGCTTTAGTCCA	AATCCAACCAAGTCCGTAGC	50.0	50.0	58.0	57.9	707-807 bp	1.0	86.3
trnS-GCU-trnL-UAA_p4	AATCCGACGCTTTAGTCCAC	AATCCAACCAAGTCCGTAGC	50.0	50.0	58.0	57.9	706-806 bp	1.0	86.3
trnS-GCU-trnL-UAA_p5	CAATCCGACGCTTTAGTCCA	CCGTAGCGTCTACCAATTCC	50.0	55.0	58.0	58.2	694-794 bp	1.0	86.0