Hotspot

atpH-atpF

LSC · 42555-43043 · 489 bp

Back to Pulsatilla

Pi 0.0126

MarkerSeek score 66.70

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 15
pi: 0.0126
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	ATTTATGGACTGGTCGTGGC	CAGTGACCCAAGGAAACGAA	50.0	50.0	58.0	58.0	578-627 bp	1.0	85.4
atpH-atpF_p2	TCGTGGCATTAGCGCTTTTA	CAGTGACCCAAGGAAACGAA	45.0	50.0	58.3	58.0	565-614 bp	1.0	83.6
atpH-atpF_p3	ATTTATGGACTGGTCGTGGC	TTAAATCCGAAACTCCCGGC	50.0	50.0	58.0	58.3	605-654 bp	1.0	83.3
atpH-atpF_p4	TCGTGGCATTAGCGCTTTTA	TTAAATCCGAAACTCCCGGC	45.0	50.0	58.3	58.3	592-641 bp	1.0	81.6
atpH-atpF_p5	ATTTATGGACTGGTCGTGGC	AGTGACCCAAGGAAACGAAA	50.0	45.0	58.0	57.3	577-626 bp	1.0	79.9