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psbM-petN

LSC · 25948-27174 · 1227 bp

Back to Pulsatilla

Pi 0.00519

MarkerSeek score 56.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1227
variable_sites: 17
pi: 0.00519
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.6	58.0	1370-1400 bp	1.0	71.9
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	TCGGGGGCATAATTCACATG	42.9	50.0	57.6	58.0	1401-1431 bp	1.0	71.7
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	AGTAAGTCTTGCTTGGGCTG	42.9	50.0	57.6	57.8	1376-1406 bp	1.0	70.4
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	ATGGGGAAGAAGTGGACTCT	42.9	50.0	57.6	57.6	1316-1346 bp	1.0	69.2
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	GGGGGCATAATTCACATGGA	42.9	50.0	57.6	57.6	1399-1429 bp	1.0	68.8