Hotspot

trnY-GUA-trnD-GUC

LSC · 24057-24652 · 596 bp

Back to Pulsatilla

Pi 0.0614
MarkerSeek score 87.60
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
596
variable_sites
83
pi
0.0614
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.93
markerseek_score
87.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 TGGCAATATGTCTACGCTGG GTGCTCTGACCGATTGAACT 50.0 50.0 57.8 57.9 568-883 bp 1.0 89.4
trnY-GUA-trnD-GUC_p2 GGCAATATGTCTACGCTGGT GTGCTCTGACCGATTGAACT 50.0 50.0 57.8 57.9 567-882 bp 1.0 89.4
trnY-GUA-trnD-GUC_p3 GGGGACGGACTGTAAATTCG GTGCTCTGACCGATTGAACT 55.0 50.0 58.4 57.9 589-904 bp 1.0 88.5
trnY-GUA-trnD-GUC_p4 GGGACGGACTGTAAATTCGT GTGCTCTGACCGATTGAACT 50.0 50.0 57.6 57.9 588-903 bp 1.0 88.3
trnY-GUA-trnD-GUC_p5 GATTCTTCCTGGGTCGATGC GTGCTCTGACCGATTGAACT 55.0 50.0 58.4 57.9 622-937 bp 1.0 88.0