Hotspot

rps4-rps16

LSC · 632-1318 · 687 bp

Back to Pulsatilla

Pi 0.0225
MarkerSeek score 57.60
Primer available yes
Label rps4-rps16

Feature Metadata

label_name
rps4-rps16
feature_type
igs
region
LSC
length_bp
687
variable_sites
37
pi
0.0225
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-rps16_p1 AGTCGTAGAATATTATTCCCGTCA ATTCCTTCAAGTCGCACGTT 37.5 45.0 57.1 58.1 698-1040 bp 1.0 47.5
rps4-rps16_p2 AGTCGTAGAATATTATTCCCGTCA AAGTCGCACGTTGCTTTCTA 37.5 45.0 57.1 58.1 690-1032 bp 1.0 47.5
rps4-rps16_p3 GTCGTAGAATATTATTCCCGTCA ATTCCTTCAAGTCGCACGTT 39.1 45.0 55.8 58.1 697-1039 bp 1.0 47.5
rps4-rps16_p4 GTCGTAGAATATTATTCCCGTCA AAGTCGCACGTTGCTTTCTA 39.1 45.0 55.8 58.1 689-1031 bp 1.0 47.5
rps4-rps16_p5 AGTCGTAGAATATTATTCCCGTCAA ATTCCTTCAAGTCGCACGTT 36.0 45.0 57.7 58.1 698-1040 bp 1.0 47.5