Hotspot

rpl16

LSC · 78106-79611 · 1506 bp

Back to Pulmonaria

Pi 0.00133
MarkerSeek score 59.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1506
variable_sites
2
pi
0.00133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TGTTTGTCCAAAAGGAAACCTG TCCTTATCTTTTCCTTCTATCCA 40.9 34.8 57.5 54.2 1692 bp 1.0 40.1
rpl16_p2 TGTTTGTCCAAAAGGAAACCTG GGGGTTGAATAAAAATTAGATTGACC 40.9 34.6 57.5 57.1 1620 bp 1.0 40.1
rpl16_p3 TGTTTGTCCAAAAGGAAACCTG TCCTTATCTTTTCCTTCTATCCAA 40.9 33.3 57.5 55.0 1692 bp 1.0 40.1
rpl16_p4 TGTTTGTCCAAAAGGAAACCTG TTCCTTATCTTTTCCTTCTATCCA 40.9 33.3 57.5 55.0 1693 bp 1.0 40.1
rpl16_p5 GTTTGTCCAAAAGGAAACCTGA TCCTTATCTTTTCCTTCTATCCA 40.9 34.8 57.3 54.2 1691 bp 1.0 40.1