Hotspot

ndhA

SSC · 115480-117653 · 2174 bp

Back to Pulmonaria

Pi 0.00138

MarkerSeek score 55.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2174
variable_sites: 3
pi: 0.00138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTCAGTTACCATAGGGAACA	GCCCGCCAGGTTTTATCAAT	42.9	50.0	55.7	58.9	2390-2391 bp	1.0	46.6
ndhA_p2	AACTCAGTTACCATAGGGAACA	GCCCGCCAGGTTTTATCAAT	40.9	50.0	56.5	58.9	2391-2392 bp	1.0	44.7
ndhA_p3	ACTCAGTTACCATAGGGAACAT	GCCCGCCAGGTTTTATCAAT	40.9	50.0	56.2	58.9	2390-2391 bp	1.0	43.0
ndhA_p4	AAACTCAGTTACCATAGGGAACA	GCCCGCCAGGTTTTATCAAT	39.1	50.0	57.2	58.9	2392-2393 bp	1.0	42.3
ndhA_p5	ACTCAGTTACCATAGGGAACA	GCCCGCCAGGTTTTATCAA	42.9	52.6	55.7	58.4	2390-2391 bp	1.0	42.3