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psbE-petL

LSC · 62057-63110 · 1054 bp

Back to Pulmonaria

Pi 0.00095

MarkerSeek score 59.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1054
variable_sites: 1
pi: 0.00095
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1198-1199 bp	1.0	79.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1200-1201 bp	1.0	79.7
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1121-1122 bp	1.0	78.9
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.8	58.0	1123-1124 bp	1.0	78.8
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.2	58.0	1207-1208 bp	1.0	78.8