Hotspot

ycf3-trnS-GGA

LSC · 43655-44446 · 792 bp

Back to Pulmonaria

Pi 0.00126

MarkerSeek score 59.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	955 bp	1.0	66.7
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	956 bp	1.0	66.7
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	TCGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	57.9	976 bp	1.0	66.3
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	TCGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	57.9	977 bp	1.0	66.3
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	926 bp	1.0	65.9