Hotspot

rpl16

LSC · 81065-82584 · 1520 bp

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Pi 0.00175

MarkerSeek score 38.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1520
variable_sites: 4
pi: 0.00175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCTTCTTTTAATGTAAAGGACGA	ATTTCCATCAAAACTCCTTTG	32.0	33.3	56.3	52.5	1664-1665 bp	1.0	40.3
rpl16_p2	TCTCTTCTTTTAATGTAAAGGACGA	AATTTCCATCAAAACTCCTTTG	32.0	31.8	56.3	53.4	1665-1666 bp	1.0	40.3
rpl16_p3	TCTTCTTTTAATGTAAAGGACGAAGT	ATTTCCATCAAAACTCCTTTG	30.8	33.3	57.1	52.5	1662-1663 bp	1.0	40.3
rpl16_p4	TCTTCTTTTAATGTAAAGGACGAAGT	AATTTCCATCAAAACTCCTTTG	30.8	31.8	57.1	53.4	1663-1664 bp	1.0	40.3
rpl16_p5	CTCTTCTTTTAATGTAAAGGACGAAGT	ATTTCCATCAAAACTCCTTTG	33.3	33.3	58.0	52.5	1663-1664 bp	1.0	40.3