Hotspot

psbC

LSC · 33702-35123 · 1422 bp

Back to Pugionium

Pi 0

MarkerSeek score 31.90

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCGTTTCCCAGGAAATCCG	AGCCGAGCCAGAACAAATTA	50.0	45.0	58.2	57.5	1629 bp	1.0	74.6
psbC_p2	AGGTATTCGTGCTTGGATGG	AGCCGAGCCAGAACAAATTA	50.0	45.0	57.7	57.5	1552 bp	1.0	73.4
psbC_p3	TTCGTTTCCCAGGAAATCCG	GCCGAGCCAGAACAAATTAG	50.0	50.0	58.2	57.2	1628 bp	1.0	71.9
psbC_p4	AGGTATTCGTGCTTGGATGG	GCCGAGCCAGAACAAATTAG	50.0	50.0	57.7	57.2	1551 bp	1.0	70.7
psbC_p5	TGACTTCGTTTCCCAGGAAA	AGCCGAGCCAGAACAAATTA	45.0	45.0	57.0	57.5	1633 bp	1.0	68.0