Hotspot

petB

LSC · 74725-76167 · 1443 bp

Back to Pugionium

Pi 0.00185

MarkerSeek score 60.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1443
variable_sites: 4
pi: 0.00185
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CTCCTTTGGTAGTTCGACCG	ACTATCGTTCCTTCACCAAGT	55.0	42.9	58.0	56.7	1557-1562 bp	1.0	61.8
petB_p2	CTCCTTTGGTAGTTCGACCG	ACTATCGTTCCTTCACCAAG	55.0	45.0	58.0	54.8	1557-1562 bp	1.0	54.8
petB_p3	CTCCTTTGGTAGTTCGACCG	CTATCGTTCCTTCACCAAGT	55.0	45.0	58.0	54.8	1556-1561 bp	1.0	54.8
petB_p4	CTCCTTTGGTAGTTCGACCG	ACTATCGTTCCTTCACCAAGTA	55.0	40.9	58.0	56.6	1557-1562 bp	1.0	53.0
petB_p5	CTCCTTTGGTAGTTCGACCG	TACTATCGTTCCTTCACCAAGT	55.0	40.9	58.0	56.6	1558-1563 bp	1.0	53.0