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atpE

LSC · 52054-52452 · 399 bp

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Pi 0.00334

MarkerSeek score 56.70

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: LSC
length_bp: 399
variable_sites: 2
pi: 0.00334
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	ACACGTCAGCTAGTTACAAA	CATCGATGAAGCTACTGCGA	40.0	50.0	54.7	57.9	477 bp	1.0	52.8
atpE_p2	ACACGTCAGCTAGTTACAAA	ACATCGATGAAGCTACTGCG	40.0	50.0	54.7	58.2	478 bp	1.0	52.5
atpE_p3	ACACGTCAGCTAGTTACAAA	TCGATGAAGCTACTGCGAAG	40.0	50.0	54.7	57.8	475 bp	1.0	52.2
atpE_p4	ACACGTCAGCTAGTTACAAA	CTGCGAAGGCTACGAACTTA	40.0	50.0	54.7	57.7	463 bp	1.0	51.6
atpE_p5	ACACGTCAGCTAGTTACAAA	TGCGAAGGCTACGAACTTAG	40.0	50.0	54.7	57.7	462 bp	1.0	51.6