Hotspot

rpl32-trnL

SSC · 113716-114637 · 922 bp

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Pi 0.00362

MarkerSeek score 48.10

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 922
variable_sites: 5
pi: 0.00362
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGAAGGGATATTGGACATCGT	TCGAACCGAGATGCTTTAGC	47.6	50.0	57.2	58.1	933-1097 bp	1.0	69.6
rpl32-trnL_p2	GGAAGGGATATTGGACATCGT	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.2	57.9	907-1071 bp	1.0	69.2
rpl32-trnL_p3	GGAAGGGATATTGGACATCGT	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.2	57.8	900-1064 bp	1.0	68.7
rpl32-trnL_p4	GGAAGGGATATTGGACATCGT	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.2	57.8	906-1070 bp	1.0	68.7
rpl32-trnL_p5	GGAAGGGATATTGGACATCGT	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.2	57.8	905-1069 bp	1.0	68.7