Hotspot

rbcL-accD

LSC · 56186-56900 · 715 bp

Back to Pugionium

Pi 0.00187

MarkerSeek score 60.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 2
pi: 0.00187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	TAGCTCGCCTCTGTATTCCA	55.0	50.0	57.7	57.9	864-866 bp	1.0	77.7
rbcL-accD_p2	GCTTGCAAATGGAGTCCTGA	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.5	57.9	875-877 bp	1.0	76.3
rbcL-accD_p3	TGAGGCTTGCAAATGGAGTC	TAGCTCGCCTCTGTATTCCA	50.0	50.0	58.5	57.9	879-881 bp	1.0	76.3
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	GCTCGCCTCTGTATTCCAAT	55.0	50.0	57.7	57.8	862-864 bp	1.0	76.2
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	TGCTTTACTTAGCTCGCCTC	55.0	50.0	57.7	57.7	873-875 bp	1.0	75.9