Hotspot

psbC-trnS

LSC · 35124-35303 · 180 bp

Back to Pugionium

Pi 0.0259

MarkerSeek score 56.50

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 7
pi: 0.0259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	363 bp	1.0	78.1
psbC-trnS_p2	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	313 bp	1.0	78.0
psbC-trnS_p3	CGGCAGCAGGATTTGAAAAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.6	58.2	324 bp	1.0	76.8
psbC-trnS_p4	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	274 bp	1.0	76.8
psbC-trnS_p5	TTCGTGGGTCATTTATGGCA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.5	58.2	364 bp	1.0	76.1