Hotspot

trnD-trnY

LSC · 29906-30343 · 438 bp

Back to Pugionium

Pi 0.00609

MarkerSeek score 39.90

Primer available yes

Label trnD-trnY

Feature Metadata

label_name: trnD-trnY
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 4
pi: 0.00609
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-trnY_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	508 bp	1.0	74.9
trnD-trnY_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	507 bp	1.0	74.9
trnD-trnY_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	529 bp	1.0	74.0
trnD-trnY_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	528 bp	1.0	73.8
trnD-trnY_p5	TGTTTATTGAATCCGCCGGG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.6	57.8	568 bp	1.0	73.7