Hotspot

trnR-atpA

LSC · 9560-9856 · 297 bp

Back to Pugionium

Pi 0.00673

MarkerSeek score 59.10

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 297
variable_sites: 3
pi: 0.00673
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	GCGAAAAGCGTCCATTGTC	TCAAGAGCAACTGGAACGTT	52.6	45.0	58.0	57.7	417-423 bp	1.0	71.1
trnR-atpA_p2	GCGAAAAGCGTCCATTGTC	TTCAAGAGCAACTGGAACGT	52.6	45.0	58.0	57.7	418-424 bp	1.0	71.1
trnR-atpA_p3	GCGAAAAGCGTCCATTGTC	ACATTAACCGCTGAAGCAGA	52.6	45.0	58.0	57.5	458-464 bp	1.0	69.8
trnR-atpA_p4	GCGAAAAGCGTCCATTGTC	AGACATTAACCGCTGAAGCA	52.6	45.0	58.0	57.5	460-466 bp	1.0	69.8
trnR-atpA_p5	GCGAAAAGCGTCCATTGTCT	TCAAGAGCAACTGGAACGTT	50.0	45.0	59.5	57.7	417-423 bp	1.0	67.2