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trnK-rps16

LSC · 4318-5049 · 732 bp

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Pi 0.00455

MarkerSeek score 61.70

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: LSC
length_bp: 732
variable_sites: 5
pi: 0.00455
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	AACTCTACCGCAGATCTGGA	CGCTCAACCAACAAGAACAG	50.0	50.0	57.9	57.6	904-905 bp	1.0	76.2
trnK-rps16_p2	AGTCGCACTTAAAAGCCGAG	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	847-848 bp	1.0	72.8
trnK-rps16_p3	TACCGCAGATCTGGACGAAT	CGCTCAACCAACAAGAACAG	50.0	50.0	58.6	57.6	899-900 bp	1.0	72.5
trnK-rps16_p4	ACTCTACCGCAGATCTGGAC	CGCTCAACCAACAAGAACAG	55.0	50.0	58.6	57.6	903-904 bp	1.0	72.5
trnK-rps16_p5	GCAGATCTGGACGAATCCTT	CGCTCAACCAACAAGAACAG	50.0	50.0	57.4	57.6	895-896 bp	1.0	72.4