Hotspot

ycf1

SSC · 122611-127356 · 4746 bp

Back to Pueraria

Pi 0.00601

MarkerSeek score 33.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4746
variable_sites: 71
pi: 0.00601
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGAGAGGTTTTTGAACAAAC	GGAAGAATACCCCCTCCCTT	38.1	55.0	55.2	58.1	215-242 bp	1.0	52.0
ycf1_p2	TGTTTACGAGAGGTTTTTGAACA	GGAAGAATACCCCCTCCCTT	34.8	55.0	56.9	58.1	220-247 bp	1.0	49.2
ycf1_p3	ACGAGAGGTTTTTGAACAAA	GGAAGAATACCCCCTCCCTT	35.0	55.0	53.6	58.1	215-242 bp	1.0	46.9
ycf1_p4	TGTTTACGAGAGGTTTTTGAACAA	GGAAGAATACCCCCTCCCTT	33.3	55.0	57.5	58.1	220-247 bp	1.0	46.3
ycf1_p5	ACGAGAGGTTTTTGAACAAAC	TAGGAAGAATACCCCCTCCC	38.1	55.0	55.2	57.2	217-244 bp	1.0	46.2