Hotspot

ndhF-rpl32

SSC · 111790-112274 · 485 bp

Back to Pueraria

Pi 0.00788

MarkerSeek score 48.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 485
variable_sites: 9
pi: 0.00788
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGTTGCTGTCGGAAAAAGT	TCTTTTTCCATAAGGTGTTCCGA	45.0	39.1	58.2	58.0	677-680 bp	1.0	54.9
ndhF-rpl32_p2	CCCACATACGGCGAAGATTT	TCTTTTTCCATAAGGTGTTCCGA	50.0	39.1	58.3	58.0	698-701 bp	1.0	54.1
ndhF-rpl32_p3	ACGGCGAAGATTTTTGGTTG	TCTTTTTCCATAAGGTGTTCCGA	45.0	39.1	57.6	58.0	691-694 bp	1.0	53.5
ndhF-rpl32_p4	GGCGAAGATTTTTGGTTGCT	TCTTTTTCCATAAGGTGTTCCGA	45.0	39.1	57.6	58.0	689-692 bp	1.0	53.3
ndhF-rpl32_p5	TGGTTGCTGTCGGAAAAAGT	TCCCTTCTTTTTCCATAAGGTGT	45.0	39.1	58.2	57.7	682-685 bp	1.0	52.4