Hotspot

rps3-rps19

LSC · 83385-83739 · 355 bp

Back to Pueraria

Pi 0.02

MarkerSeek score 46.10

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 18
pi: 0.02
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	ACTCCTTTTGTACCCGCTTG	TAAACTTCCGAGGACATGCG	50.0	50.0	58.1	58.0	881-902 bp	1.0	81.0
rps3-rps19_p2	CTCCTTTTGTACCCGCTTGT	TAAACTTCCGAGGACATGCG	50.0	50.0	58.1	58.0	880-901 bp	1.0	81.0
rps3-rps19_p3	TGAACTCCTTTTGTACCCGC	TAAACTTCCGAGGACATGCG	50.0	50.0	58.1	58.0	884-905 bp	1.0	80.9
rps3-rps19_p4	TGATCCATTCAACACGTGCA	TAAACTTCCGAGGACATGCG	45.0	50.0	58.1	58.0	940-961 bp	1.0	80.8
rps3-rps19_p5	CTCCTTTTGTACCCGCTTGT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.1	58.1	1285-1306 bp	1.0	80.1