Hotspot

trnQ-UUG-rps16

LSC · 55477-55979 · 503 bp

Back to Pueraria

Pi 0.012

MarkerSeek score 51.40

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 14
pi: 0.012
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAGGTTCGAATCCTTCCGTC	GTCTTTCAAGTCGCACGTTG	55.0	50.0	58.1	58.0	561-583 bp	1.0	84.4
trnQ-UUG-rps16_p2	GAGGTTCGAATCCTTCCGTC	ATGTCTTTCAAGTCGCACGT	55.0	45.0	58.1	58.1	563-585 bp	1.0	83.4
trnQ-UUG-rps16_p3	GAGGTTCGAATCCTTCCGTC	GCACGTTGCTTTTTACCACA	55.0	45.0	58.1	57.8	549-571 bp	1.0	83.0
trnQ-UUG-rps16_p4	GAGGTTCGAATCCTTCCGTC	CATGTCTTTCAAGTCGCACG	55.0	50.0	58.1	57.8	564-586 bp	1.0	82.7
trnQ-UUG-rps16_p5	AAAATCGAAAATGGGGCGTG	GTCTTTCAAGTCGCACGTTG	45.0	50.0	57.6	58.0	620-642 bp	1.0	81.6