Hotspot

trnC-GCA-rpoB

LSC · 33905-34904 · 1000 bp

Back to Pueraria

Pi 0.00407

MarkerSeek score 53.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 10
pi: 0.00407
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	CCTTCTGTTAAGCCCCGATC	50.0	55.0	58.1	58.0	1157-1163 bp	1.0	80.6
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	AAAGCCCTTCTGTTAAGCCC	50.0	50.0	58.1	58.1	1162-1168 bp	1.0	80.3
trnC-GCA-rpoB_p3	AAAAGGATTTGCAGTCCCCC	CCTTCTGTTAAGCCCCGATC	50.0	55.0	58.4	58.0	1139-1145 bp	1.0	78.4
trnC-GCA-rpoB_p4	CCGGATTTGAACTGGGGAAA	TAAGCCCCGATCAATGAACC	50.0	50.0	58.1	57.7	1149-1155 bp	1.0	78.2
trnC-GCA-rpoB_p5	AAAAGGATTTGCAGTCCCCC	AAAGCCCTTCTGTTAAGCCC	50.0	50.0	58.4	58.1	1144-1150 bp	1.0	78.2