Hotspot

trnT-GGU-trnE-UUC

LSC · 29849-30693 · 845 bp

Back to Pueraria

Pi 0.00607

MarkerSeek score 52.40

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 12
pi: 0.00607
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	AACTTCCTTTCGTAGCACCC	50.0	50.0	58.0	57.8	963-993 bp	1.0	82.0
trnT-GGU-trnE-UUC_p2	CCATGGCGTTACTCTACCAC	AACTTCCTTTCGTAGCACCC	55.0	50.0	58.1	57.8	942-972 bp	1.0	81.6
trnT-GGU-trnE-UUC_p3	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	918-948 bp	1.0	81.5
trnT-GGU-trnE-UUC_p4	TGGCGTTACTCTACCACTGA	AACTTCCTTTCGTAGCACCC	50.0	50.0	58.1	57.8	939-969 bp	1.0	81.4
trnT-GGU-trnE-UUC_p5	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	897-927 bp	1.0	81.4