Hotspot

ycf3-psaA

LSC · 18524-19338 · 815 bp

Back to Pueraria

Pi 0.00539

MarkerSeek score 54.80

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 10
pi: 0.00539
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	AAGTTGCGGAGTCTTGGTTT	TTTCACTTCTGGTTCCGGTG	45.0	50.0	57.9	58.0	960-969 bp	1.0	81.3
ycf3-psaA_p2	AAGTTGCGGAGTCTTGGTTT	AATGACCAGGTTTTGCCCAT	45.0	45.0	57.9	58.0	1021-1030 bp	1.0	81.2
ycf3-psaA_p3	AAGTTGCGGAGTCTTGGTTT	ATGACCAGGTTTTGCCCATT	45.0	45.0	57.9	58.0	1020-1029 bp	1.0	81.2
ycf3-psaA_p4	TTGGTTGAAGATCACAGGGC	AATGACCAGGTTTTGCCCAT	50.0	45.0	58.1	58.0	933-942 bp	1.0	81.1
ycf3-psaA_p5	TTGGTTGAAGATCACAGGGC	ATGACCAGGTTTTGCCCATT	50.0	45.0	58.1	58.0	932-941 bp	1.0	81.1