Hotspot

trnK-UUU-rbcL

LSC · 4290-5306 · 1017 bp

Back to Pueraria

Pi 0.00741
MarkerSeek score 57.70
Primer available yes
Label trnK-UUU-rbcL

Feature Metadata

label_name
trnK-UUU-rbcL
feature_type
igs
region
LSC
length_bp
1017
variable_sites
19
pi
0.00741
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rbcL_p1 AGAGTCGCTCTTAAAAGCCG AGTCCTGAATTAGCTGCTGC 50.0 50.0 58.0 58.0 1125-1139 bp 1.0 83.1
trnK-UUU-rbcL_p2 AGTCGCTCTTAAAAGCCGAG AGTCCTGAATTAGCTGCTGC 50.0 50.0 58.0 58.0 1123-1137 bp 1.0 83.1
trnK-UUU-rbcL_p3 AGAGTCGCTCTTAAAAGCCG GAGGCTAGCAAATGGAGTCC 50.0 55.0 58.0 58.0 1140-1154 bp 1.0 83.0
trnK-UUU-rbcL_p4 AGTCGCTCTTAAAAGCCGAG GAGGCTAGCAAATGGAGTCC 50.0 55.0 58.0 58.0 1138-1152 bp 1.0 83.0
trnK-UUU-rbcL_p5 GAGTCGCTCTTAAAAGCCGA AGTCCTGAATTAGCTGCTGC 50.0 50.0 58.0 58.0 1124-1138 bp 1.0 83.0