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trnH-GUG-psbA

LSC · 80-304 · 225 bp

Back to Pueraria

Pi 0.019

MarkerSeek score 55.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 10
pi: 0.019
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCCTCTAGATCTAGCTGCGA	55.0	55.0	58.1	57.8	288-308 bp	1.0	81.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	TCCCTCTAGATCTAGCTGCG	55.0	55.0	58.1	57.8	289-309 bp	1.0	81.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	CCCTCTAGATCTAGCTGCGA	50.0	55.0	57.9	57.8	302-322 bp	1.0	81.2
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	TCCCTCTAGATCTAGCTGCG	50.0	55.0	57.9	57.8	303-323 bp	1.0	81.2
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	CCCTCTAGATCTAGCTGCGA	50.0	55.0	57.9	57.8	301-321 bp	1.0	81.2