Hotspot

rps3

LSC · 83878-84540 · 663 bp

Back to Pterygiella

Pi 0.00302

MarkerSeek score 60.50

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 2
pi: 0.00302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	ACGAATGAACGAGTTTCCCC	TGCTGATGTGTATAGTAATGGGG	50.0	43.5	58.2	57.8	714 bp	1.0	52.8
rps3_p2	ACGAATGAACGAGTTTCCCC	GCTGATGTGTATAGTAATGGGGT	50.0	43.5	58.2	57.8	713 bp	1.0	52.8
rps3_p3	GGTCCGAAAACGAATGAACG	TGCTGATGTGTATAGTAATGGGG	50.0	43.5	57.8	57.8	723 bp	1.0	52.6
rps3_p4	GGTCCGAAAACGAATGAACG	GCTGATGTGTATAGTAATGGGGT	50.0	43.5	57.8	57.8	722 bp	1.0	52.6
rps3_p5	GAATGAACGAGTTTCCCCCT	TGCTGATGTGTATAGTAATGGGG	50.0	43.5	57.5	57.8	712 bp	1.0	50.5