Hotspot

petB

LSC · 76228-77603 · 1376 bp

Back to Pterygiella

Pi 0.00293

MarkerSeek score 61.20

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1376
variable_sites: 4
pi: 0.00293
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AAGAACCACAAAGGCCTAGC	TCCTCCTCTAAGTGATAAATCGT	50.0	39.1	58.1	56.3	1536-1547 bp	1.0	42.4
petB_p2	GAACCACAAAGGCCTAGCTT	TCCTCCTCTAAGTGATAAATCGT	50.0	39.1	58.1	56.3	1534-1545 bp	1.0	42.4
petB_p3	AAGAACCACAAAGGCCTAGC	TCCTCTAAGTGATAAATCGTTGGA	50.0	37.5	58.1	57.2	1533-1544 bp	1.0	41.8
petB_p4	GAACCACAAAGGCCTAGCTT	TCCTCTAAGTGATAAATCGTTGGA	50.0	37.5	58.1	57.2	1531-1542 bp	1.0	41.8
petB_p5	ACCACAAAGGCCTAGCTTTT	TCCTCCTCTAAGTGATAAATCGT	45.0	39.1	57.6	56.3	1532-1543 bp	1.0	40.8